Capture a broad range of transcripts from coding to noncoding RNA
Whole Transcriptome Sequencing (WTS)
Clinical Enterprise leverages advanced Illumina technology to offer bespoke Whole Transcriptome Sequencing (WTS) services. Specializing in customized RNA sequencing (RNASeq) studies, our WTS is ideal for detailed gene expression analysis and innovative RNA research projects, including those using in vitro transcribed (IVT) RNA. With a commitment to flexibility and precision, we enable researchers and healthcare professionals to dissect complex RNA landscapes and obtain actionable insights, fostering groundbreaking discoveries in their fields.
- Customized Solutions: Each project is uniquely designed to meet the specific research questions and experimental conditions of our clients, from routine analyses to complex novel RNA studies.
- Advanced Technology: Utilizing the latest advancements from Illumina ensures high-quality sequencing results with each tailored project.
- Diverse RNA Analysis Capabilities: We handle a variety of RNA types and offer comprehensive solutions, including the analysis of IVT RNA and other specialized RNA categories.
Applications of Whole Transcriptome Sequencing
Whole Transcriptome Sequencing is used extensively in various innovative applications, making it an essential tool for researchers.
Targeted RNA Studies
Tailored for specific RNA types like mRNA, non-coding RNA, or precursors, providing deep insights into RNA pathway processing and function.
Transcriptome-Wide Analysis
From gene expression profiling to the discovery of novel transcripts and splice variants, our services facilitate a comprehensive understanding of transcriptomic dynamics.
Specialized Research Applications
Ideal for custom studies such as drug response evaluations, genetic research, and developmental biology studies.
Service Offerings
Clinical Enterprise provides flexible WTS options for your next project.
Bespoke Transcriptome Services
We customize our sequencing and analysis to suit the exact needs of each project, from small-scale targeted studies to large-scale transcriptomic surveys, providing you with a dynamic scale.
Flexible Sequencing Configurations
We offer varying read lengths and depths to capture precise RNA dynamics across different conditions and treatments, providing comprehensive analysis of transcriptional regulation network.
Sample Requirements
- Sample Types: We accept a broad range of RNA types, including fragile and specialized samples like IVT RNA.
- Volume and Concentration: Minimum requirements can vary; please contact us to discuss the specifics of your project.
- Shipping: Customized shipping protocols are based on sample type and project needs.
Technology
Clinical Enterprise utilizes robust and cutting-edge platforms to extract, prepare, and sequence samples.
Extraction and Preparation
Utilizing advanced RNA methods ensures optimal quality and integrity for a wide variety of RNA samples, facilitating high-quality library preparation using the Illumina Stranded Total RNA Kit with Ribo-Zero.
Illumina Stranded Total RNA Prep with Ribo-Zero Plus workflow
Next Generation Sequencing
Our sequencing services ensure high-quality data output with options tailored for each project.
NovaSeq 6000
Employing the Illumina NovaSeq 6000, our service provides adaptable sequencing options tailored for comprehensive coverage and high-resolution insights, ideal for both focused and extensive transcriptome analyses.
Advanced Workflow and Bioinformatics
Our sophisticated bioinformatics setup incorporates the DRAGEN RNA v4.2.4 platform, enhancing our capability to deliver precise and rapid RNASeq data analysis.
Variant Calls and Gene/Transcript Quantification
Accurate detection and quantification of genetic variants and transcript levels
Analysis of Splice Sites and Fusion Genes
Detailed examination of RNA splicing patterns and fusion events identification
Customizable Analysis
Differential expression analysis, regulatory element mapping, and more
Data Delivery and Reporting
- Standard Deliverables: Include comprehensive outputs such as variant calls, transcript counts, splice site identification, fusion gene data, and thorough QC reports.
- Custom Reports: Tailored to the project’s specifications, providing in-depth analysis and insights specific to the client’s research goals.